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Publications 2005 - 2009

Chemical Protein Engineering: Synthetic and Semisynthetic Peptides and Proteins
Citation key 046.2008.merkel
Author Merkel, L. and Moroder, L. and Budisa
Title of Book Protein Engineering
Pages 29-64
Year 2008
ISBN 978-3-540-70937-4
DOI 10.1007/978-3-540-70941-1_2
Location Berlin, Heidelberg, New York
Editor T. RajBhandary and C. Koehrer
Publisher Springer Verlag
Abstract Chemical engineering of proteins provides a pool of various synthetic and semisynthetic methods. These involve techniques for the design of peptides and proteins equipped with chemical handles at defined positions in the target structures. Using water-compatible bioorthogonal chemical ligations gained immense importance in chemical and cellular biology for regioselective addressing to target structures. Beside classical protein modifications, we present examples not only of expressed protein modifications, but also of the attachment of small molecules by native and expressed chemical ligation, modified Staudinger ligation, and of the copper(I)-catalyzed Huisgen [3+2] cycloaddition of azides and alkynes. Recently, in addition to enzymatic methods, the use of organometallic chemistry, i.e. regioselective palladium-catalyzed C-C coupling (Sonogashira, Suzuki, and Mizoroki-Heck reactions), became available as a tool for tailored protein modifications. This chapter reviews all traditional and newly developed chemoselective modifications and ligation methods in the field.
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