direkt zum Inhalt springen

direkt zum Hauptnavigationsmenü

Sie sind hier

TU Berlin

Page Content


An expanded genetic code for probing the role of electrostatics in enzyme catalysis by vibrational Stark spectroscopy
Citation key 159.2017.Völler
Author Völler J.-S., and Biava, H., and Hildebrandt, P., and Budisa, N.
Year 2017
DOI doi: 10.1016/j.bbagen.2017.02.009.doi.org
Journal Biochim. Biophys. Acta
Volume [Epub ahead of print]
Abstract BACKGROUND: To find experimental validation for electrostatic interactions essential for catalytic reactions represents a challenge due to practical limitations in assessing electric fields within protein structures. SCOPE OF REVIEW: This review examines the applications of non-canonical amino acids (ncAAs) as genetically encoded probes for studying the role of electrostatic interactions in enzyme catalysis. MAJOR CONCLUSIONS: ncAAs constitute sensitive spectroscopic probes to detect local electric fields by exploiting the vibrational Stark effect (VSE) and thus have the potential to map the protein electrostatics. GENERAL SIGNIFICANCE: Mapping the electrostatics in proteins will improve our understanding of natural catalytic processes and, in beyond, will be helpful for biocatalyst engineering. This article is part of a Special Issue entitled "Biochemistry of Synthetic Biology - Recent Developments" Guest Editor: Dr. Ilka Heinemann and Dr. Patrick O'Donoghue. Copyright © 2017 Elsevier B.V. All rights reserved.
Link to publication Link to original publication Download Bibtex entry

Zusatzinformationen / Extras

Quick Access:

Schnellnavigation zur Seite über Nummerneingabe

Auxiliary Functions

This site uses Piwik for anonymized webanalysis. Visit Data Privacy for more information and opt-out options.