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Efficient N-terminal Glycoconjugation of Proteins by the N-End Rule
Citation key 049.2008.merkel
Author Merkel, L. and Beckmann, H. S. G. and Wittmann, V. and Budisa, N.
Pages 1220-1224
Year 2008
DOI 10.1002/cbic.200800050
Journal ChemBioChem
Volume 9
Number 8
Abstract Bulky amino acids in positions 2 and 3 of proteins protect both Met and noncanonical azidohomoalanine, introduced by the auxotrophy-based method, from being excised by the enzymes responsible for N-terminal Met excision. Bioorthogonal transformation enables new specific functionalization of target proteins. We validated this general concept by designing an N-terminal glycoconjugated barstar capable of lectin binding without losing its biological activity.
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