Institut für Chemie: Publications 2005

Publications 2005 - 2009

Chemical Protein Engineering: Synthetic and Semisynthetic Peptides and Proteins
Citation key	046.2008.merkel
Author	Merkel, L. and Moroder, L. and Budisa
Title of Book	Protein Engineering
Pages	29-64
Year	2008
ISBN	978-3-540-70937-4
DOI	10.1007/978-3-540-70941-1_2
Location	Berlin, Heidelberg, New York
Editor	T. RajBhandary and C. Koehrer
Publisher	Springer Verlag
Abstract	Chemical engineering of proteins provides a pool of various synthetic and semisynthetic methods. These involve techniques for the design of peptides and proteins equipped with chemical handles at defined positions in the target structures. Using water-compatible bioorthogonal chemical ligations gained immense importance in chemical and cellular biology for regioselective addressing to target structures. Beside classical protein modifications, we present examples not only of expressed protein modifications, but also of the attachment of small molecules by native and expressed chemical ligation, modified Staudinger ligation, and of the copper(I)-catalyzed Huisgen [3+2] cycloaddition of azides and alkynes. Recently, in addition to enzymatic methods, the use of organometallic chemistry, i.e. regioselective palladium-catalyzed C-C coupling (Sonogashira, Suzuki, and Mizoroki-Heck reactions), became available as a tool for tailored protein modifications. This chapter reviews all traditional and newly developed chemoselective modifications and ligation methods in the field.