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Publications 2010 - 2012

Performance analysis of orthogonal pairs designed for an expanded eukaryotic genetic code
Citation key 090.2012.nehring
Author Nehring, S. and Budisa, N. and Wiltschi, B.
Pages e31992
Year 2012
DOI 10.1371/journal.pone.0031992
Journal PLoSONE
Volume 2012
Number 7
Abstract The suppression of amber stop codons with non-canonical amino acids (ncAAs) is used for the site-specific introduction of many unusual functions into proteins. Specific orthogonal aminoacyl-tRNA synthetase (o-aaRS)/amber suppressor tRNACUA pairs (o-pairs) for the incorporation of ncAAs in S. cerevisiae were previously selected from an E. coli tyrosyl-tRNA synthetase/tRNACUA mutant library. Incorporation fidelity relies on the specificity of the o-aaRSs for their ncAAs and the ability to effectively discriminate against their natural substrate Tyr or any other canonical amino acid.
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