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Publications 2013 - 2015

Chemical evolution of a bacterial proteome
Citation key 131.2015.hoesl
Author Hoesl, M. G. and Oehm, S. and Durkin, P. and Darmon, E. and Peil, L. and Aerni, H-R. and Rappsilber, J. and Rinehart, J. and Leach, D. and Söll, D. and Budisa, N.
Pages 10030-10034
Year 2015
DOI 10.1002/anie.201502868
Journal Angew. Chem. Int. Ed. Engl.
Volume 54
Number 34
Abstract We have changed the amino acid set of the genetic code of Escherichia coli by evolving cultures capable of growing on the synthetic noncanonical amino acid L-β-(thieno[3,2-b]pyrrolyl)alanine ([3,2]Tpa) as a sole surrogate for the canonical amino acid L-tryptophan (Trp). A long-term cultivation experiment in defined synthetic media resulted in the evolution of cells capable of surviving Trp→[3,2]Tpa substitutions in their proteomes in response to the 20 899 TGG codons of the E. coli W3110 genome. These evolved bacteria with new-to-nature amino acid composition showed robust growth in the complete absence of Trp. Our experimental results illustrate an approach for the evolution of synthetic cells with alternative biochemical building blocks.
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