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Publications 2013 - 2015

Improved method to retain cytosolic reporter protein fluorescence while staining for nuclear proteins
Citation key 111.2014.heinen
Author Heinen, A.P. and Wanke, F. and Moos, S. and Attig, S. and Luche, H. and Pal, P.P. and Budisa, N. and Fehling, H.J. and Waisman, A. and Kurschus, F.C.
Pages 621–627
Year 2014
DOI 10.1002/cyto.a.22451
Journal Cytometry A.
Volume 85
Number 7
Abstract Staining of transcription factors (TFs) together with retention of fluorescent reporter proteins is hindered by loss of fluorescence using current available methods. In this study, it is shown that current TF staining protocols do not destroy fluorescent proteins (FPs) but rather that fixation is not sufficient to retain FPs in the cytosol of the permeabilized cells. In this article, a simple and reliable protocol is elaborated, which allows efficient TF and cytokine staining while retaining FPs inside fixed cells. © 2014 International Society for Advancement of Cytometry
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