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Publications 2013 - 2015
| Citation key | 131.2015.hoesl |
|---|---|
| Author | Hoesl, M. G. and Oehm, S. and Durkin, P. and Darmon, E. and Peil, L. and Aerni, H-R. and Rappsilber, J. and Rinehart, J. and Leach, D. and Söll, D. and Budisa, N. |
| Pages | 10030-10034 |
| Year | 2015 |
| DOI | 10.1002/anie.201502868 |
| Journal | Angew. Chem. Int. Ed. Engl. |
| Volume | 54 |
| Number | 34 |
| Abstract | We have changed the amino acid set of the genetic code of Escherichia coli by evolving cultures capable of growing on the synthetic noncanonical amino acid L-β-(thieno[3,2-b]pyrrolyl)alanine ([3,2]Tpa) as a sole surrogate for the canonical amino acid L-tryptophan (Trp). A long-term cultivation experiment in defined synthetic media resulted in the evolution of cells capable of surviving Trp→[3,2]Tpa substitutions in their proteomes in response to the 20 899 TGG codons of the E. coli W3110 genome. These evolved bacteria with new-to-nature amino acid composition showed robust growth in the complete absence of Trp. Our experimental results illustrate an approach for the evolution of synthetic cells with alternative biochemical building blocks. |
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