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Photoactivatable mussel-based underwater adhesive proteins by an expanded genetic code
Citation key 165.2017.Hauf
Author Hauf, M., and Richter, F., and Schneider, T., and Faidt, T., and Martins, B. M., and Baumann, T., and Durkin, P., and Dobbek, H., and Jacobs, K., and Moeglich, A. and Budisa, N.
Pages 1819 - 1823
Year 2017
DOI doi: http://dx.doi.org/10.1002/cbic.201700327
Journal ChemBioChem [highlighted by cover page]
Volume 18
Abstract Marine mussels exhibit potent underwater adhesion abilities under hostile conditions employing 3,4-dihydroxy-phenylalanine (Dopa)-rich mussel-adhesive proteins (MAPs). However, their recombinant production is a major biotechnological challenge. Here, we have developed a novel strategy based on genetic code expansion by engineering efficient aminoacyl-tRNA synthetases (aaRSs) for the photocaged non-canonical amino acid (ncAA) ortho-nitrobenzyl Dopa (ONB-Dopa). The engineered ONB-DopaRS enables in vivo production of MAP type 5 site-specifically equipped with multiple instances of ONB-Dopa yielding spatiotemporally controlled underwater adhesives. Upon exposure to UV light, these proteins feature elevated wet adhesion properties highlighting a novel concept for recombinant production of photocaged bioadhesives.
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