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Publikationen 2000 - 2004

Expansion of the Genetic Code Enables Design of a Novel "Gold" Class of Green Fluorescent Proteins
Zitatschlüssel 023.2003.bae
Autor Bae, J. and Rubini, M. and Jung, G. and Wiegand, G. and Seifert, M. H. J. and Azim, M. K. and Kim, J. S. and Zumbusch, A. and Holak, T. A. and Moroder, L. and Huber, R. and Budisa, N.
Seiten 977-1202
Jahr 2003
DOI 10.1016/S0022-2836(03)00364-4
Journal J. Mol. Biol.
Jahrgang 328
Nummer 5
Zusammenfassung Much effort has been dedicated to the design of significantly red shifted variants of the green fluorescent protein (GFP) from Aequoria victora (av). These approaches have been based on classical engineering with the 20 canonical amino acids. We report here an expansion of these efforts by incorporation of an amino substituted variant of tryptophan into the "cyan" GFP mutant, which turned it into a "gold" variant. This variant possesses a red shift in emission unprecedented for any avFP, similar to "red" FPs, but with enhanced stability and a very low aggregation tendency. An increasing number of non-natural amino acids are available for chromophore redesign (by engineering of the genetic code) and enable new general strategies to generate novel classes of tailor-made GFP proteins.
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