TU Berlin

BiokatalysePublikationen 2005 - 2009

Inhalt des Dokuments

zur Navigation

Publikationen 2005 - 2009

Efficient N-terminal Glycoconjugation of Proteins by the N-End Rule
Zitatschlüssel 049.2008.merkel
Autor Merkel, L. and Beckmann, H. S. G. and Wittmann, V. and Budisa, N.
Seiten 1220-1224
Jahr 2008
DOI 10.1002/cbic.200800050
Journal ChemBioChem
Jahrgang 9
Nummer 8
Zusammenfassung Bulky amino acids in positions 2 and 3 of proteins protect both Met and noncanonical azidohomoalanine, introduced by the auxotrophy-based method, from being excised by the enzymes responsible for N-terminal Met excision. Bioorthogonal transformation enables new specific functionalization of target proteins. We validated this general concept by designing an N-terminal glycoconjugated barstar capable of lectin binding without losing its biological activity.
Link zur Originalpublikation Download Bibtex Eintrag



Schnellnavigation zur Seite über Nummerneingabe